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Ras Signaling: Methods and Protocols

Добавлено: 9-01-2014, 13:47
Ras Signaling: Methods and ProtocolsНазвание: Ras Signaling: Methods and Protocols
автор: Lorenza Trabalzini, Saverio Francesco Retta
Издательство: Humana Press
Год выпуска: 2014
ISBN: 978-1627037907
Формат: PDF
Размер: 10 MB
Количество страниц: 435
Язык: Английский

Описание: Featuring experimental approaches that shed light on the complexity of Ras GTPase biological functions, Ras Signaling: Methods and Protocols contains general overviews and detailed applications of both well-established and recently developed research techniques, including biochemical, biophysical, molecular biology, genetic and behavioral approaches, advanced high resolution fluorescence and electron microscopy imaging and “omics” technologies. Through this, the detailed volume provides information on expression, post-translational modifications, subcellular localization and dynamics, regulatory mechanisms of upstream and downstream signaling pathways and ultimately, biological activities and functions of Ras GTPases in different model systems, including high and low eukaryotic organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Wide-ranging and authoritative, Ras Signaling: Methods and Protocols serves as an aid for investigators of different backgrounds and interests related to the multiple physiological and pathological functions of the large superfamily of Ras GTPases.


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